Construction of A Decellularized Multicomponent Extracellular-matrix Interpenetrating-network Scaffold by Gelatin Microporous Hydrogel 3d Cell Culture System.

Interface tissue repair requires the construction of biomaterials with integrated structures of multiple protein types. Hydrogels that modulate internal porous structures provide a three-dimensional microenvironment for encapsulated cells, making them promise for interface tissue repair. Currently, reduction of intrinsic immunogenicity and increase of bioactive extracellular matrix secretion are issues to be considered in these materials. In this study, we used gelatin methacrylate (GelMA) hydrogel to encapsulate chondrocytes and construct a phase transition three-dimensional cell culture system (GelMA PTCC) by utilizing the thermosensitivity of gelatin microspheres to create micropores within the hydrogel. The types of bioactive extracellular matrix protein formation by chondrocytes encapsulated in hydrogels was investigated in vitro. After 28 days of culture, GelMA PTCC formed an extracellular matrix predominantly composed of collagen type II, collagen type I, and fibronectin. After decellularization, the protein types and mechanical properties were well preserved, fabricating a decellularized tissue engineered extracellular matrix interpenetrating network hydrogel (dECM-GelMA IPN) consisting of GelMA hydrogel as the first-level network and the extracellular matrix secreted by chondrocytes as the second-level network. This material has the potential to mediate the repair and regeneration of tendon-bone interface tissues with multiple protein types. This article is protected by copyright. All rights reserved.