An IncR Plasmid Harbored by a Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae Strain Possesses Five Tandem Repeats of the bla KPC-2::NTEKPC-Id Fragment.
Ning DongLizhang LiuRong ZhangKaichao ChenMiaomiao XieEdward Wai Chi ChanSheng ChenPublished in: Antimicrobial agents and chemotherapy (2019)
Completed sequences of three plasmids from a carbapenem-resistant hypervirulent Klebsiella pneumoniae isolate, SH9, were obtained. In addition to the pLVPK-like virulence-conferring plasmid (pVir-CR-HvKP_SH9), the two multidrug-resistant plasmids (pKPC-CR-HvKP4_SH9 and pCTX-M-CR-HvKP4_SH9) were predicted to originate from a single pKPC-CR-HvKP4-like multireplicon plasmid through homologous recombination. Interestingly, the bla KPC-2 gene was detectable in five tandem repeats exhibiting the format of an NTEKPC-Id-like transposon (IS26-ΔTn3-ISKpn8-bla KPC-2-ΔISKpn6-korC-orf-IS26). The data suggest an important role of DNA recombination in mediating active plasmid evolution.
Keyphrases
- klebsiella pneumoniae
- escherichia coli
- multidrug resistant
- dna repair
- dna damage
- biofilm formation
- crispr cas
- drug resistant
- acinetobacter baumannii
- gram negative
- staphylococcus aureus
- genome wide
- circulating tumor
- copy number
- dna methylation
- big data
- gene expression
- single molecule
- machine learning
- deep learning
- transcription factor
- genome wide analysis