Hydrodynamic Solvent Coupling Effects in Quartz Crystal Microbalance Measurements of Nanoparticle Deposition Kinetics.

Hydrodynamic coupling effects pertinent to quartz crystal microbalance (QCM) investigation of nanoparticle adsorption kinetics were evaluated using atomic force microscopy and the theoretical modeling. Monodisperse polymer particles of the size between 26 and 140 nm and the density of 1.05 g cm-3 were used. The ζ-potential of particles was opposite to the substrate ζ-potential that promoted their irreversible adsorption on the silica sensor. The experimental kinetic data were interpreted in terms of theoretical calculations derived from the hybrid random sequential adsorption model. This allowed us to determine the amount of hydrodynamically coupled solvent (electrolyte) for the absolute particle coverage range up to 0.5. The coupling function representing the ratio of the solvent to the particle volumes was also determined and used to explicitly calculate the solvent level in particle monolayers. It is shown that the solvent level abruptly increases with the particle coverage attaining values comparable with the particle size. One can expect that these results can serve as useful reference data for the interpretation of protein adsorption kinetics on rough surfaces where the presence of stagnant solvent is inevitable.