Antinociceptive compounds and LC-DAD-ESIMSn profile from Dictyoloma vandellianum leaves.
Lucas Silva AbreuIura Muniz AlvesRenan Fernandes do Espírito SantoYuri Mangueira do NascimentoCésar Augusto Gonçalves DantasGisele Graça Leite Dos SantosMireille Le HyaricMaria Lenise Silva GuedesMarcelo Sobral da SilvaCristiane Flora VillarrealEudes da Silva VelozoJosean Fechine TavaresPublished in: PloS one (2019)
Limonoids, quinolone alkaloids and chromones have been reported as constituents of Dictyoloma vandellianum Adr. Juss. (Rutaceae). Although those compounds are known for their biological activities, only the anti-inflammatory activity of chromones isolated from the underground parts has been evaluated. There are no studies of the pharmacological properties of the aerial parts of D. vandellianum. The present study was carried out to determine the phytochemical profile and antinociceptive activity of the methanol extract, fractions and isolated compounds of leaves of D. vandellianum. The phytochemical profile was performed by HLPC-DAD-ESIMSn and pure substances obtained were characterized by MS and NMR spectroscopy. The antinociceptive activity was assessed using the formalin assay in mice, and the motor function in the rotarod test. ME and all the fractions obtained from ME produced antinociceptive effects. Among them, the ethyl ether fraction was the most active. Data from HPLC-DAD-ESIMSn showed that the ethyl ether fraction presented 42 compounds. The major compounds isolated from this fraction-gallic acid, methyl gallate and 1,2,6-tri-O-galloyl-β-d-glucopyranose-were tested and produced antinociceptive effects. Gallic acid, methyl gallate and 1,2,6-tri-O-galloyl-β-d-glucopyranose at antinociceptive doses did not affect the motor performance in mice in the rotarod test. This work is the first report of the occurrence of gallotanins in D. vandellianum. In addition, the pharmacological study showed that D. vandellianum leaves present antinociceptive activity, probably induced by gallic acid, methyl gallate and 1,2,6-tri-O-galloyl-β-d-glucopyranose.
Keyphrases
- anti inflammatory
- ms ms
- simultaneous determination
- ionic liquid
- mass spectrometry
- risk assessment
- oxidative stress
- machine learning
- type diabetes
- metabolic syndrome
- emergency department
- high fat diet induced
- artificial intelligence
- adipose tissue
- big data
- solid phase extraction
- deep learning
- adverse drug
- single cell
- drug induced
- case control