Clinical Metagenomic Sequencing for Diagnosis of Meningitis and Encephalitis.
Michael R WilsonHannah A SampleKelsey C ZornShaun ArevaloGuixia YuJohn NeuhausScot FedermanDoug StrykeBenjamin BriggsCharles LangelierAmy BergerVanja DouglasS Andrew JosephsonFelicia C ChowBrent D FultonJoseph L DeRisiJeffrey M GelfandSamia N NaccacheJeffrey BenderJennifer Dien BardJamie MurkeyMagrit CarlsonPaul M VespaTara VijayanPaul R AllynShelley CampeauRomney M HumphriesJeffrey D KlausnerCzarina D GanzonFatemeh MemarNicolle A OcampoLara L ZimmermannStuart H CohenChristopher R PolageRoberta L DeBiasiBarbara HallerRonald DallasGabriela MaronRandall HaydenKevin MessacarSamuel R DominguezSteve MillerCharles Y ChiuPublished in: The New England journal of medicine (2019)
Routine microbiologic testing is often insufficient to detect all neuroinvasive pathogens. In this study, metagenomic NGS of CSF obtained from patients with meningitis or encephalitis improved diagnosis of neurologic infections and provided actionable information in some cases. (Funded by the National Institutes of Health and others; PDAID ClinicalTrials.gov number, NCT02910037.).