Supramolecular Nanoparticles of Histone and Hyaluronic Acid for Co-Delivery of siRNA and Photosensitizer In Vitro.
Minxing HuJianwei BaoYuanmei ZhangLele WangYa ZhangJiaxin ZhangJihui TangQianli ZouPublished in: International journal of molecular sciences (2024)
Small interfering RNA (siRNA) has significant potential as a treatment for cancer by targeting specific genes or molecular pathways involved in cancer development and progression. The addition of siRNA to other therapeutic strategies, like photodynamic therapy (PDT), can enhance the anticancer effects, providing synergistic benefits. Nevertheless, the effective delivery of siRNA into target cells remains an obstacle in cancer therapy. Herein, supramolecular nanoparticles were fabricated via the co-assembly of natural histone and hyaluronic acid for the co-delivery of HMGB1-siRNA and the photosensitizer chlorin e6 (Ce6) into the MCF-7 cell. The produced siRNA-Ce6 nanoparticles (siRNA-Ce6 NPs) have a spherical morphology and exhibit uniform distribution. In vitro experiments demonstrate that the siRNA-Ce6 NPs display good biocompatibility, enhanced cellular uptake, and improved cytotoxicity. These outcomes indicate that the nanoparticles constructed by the co-assembly of histone and hyaluronic acid hold enormous promise as a means of siRNA and photosensitizer co-delivery towards synergetic therapy.
Keyphrases
- hyaluronic acid
- photodynamic therapy
- cancer therapy
- drug delivery
- fluorescence imaging
- dna methylation
- papillary thyroid
- energy transfer
- single cell
- oxidative stress
- machine learning
- climate change
- induced apoptosis
- metabolic syndrome
- cell therapy
- cell proliferation
- insulin resistance
- young adults
- skeletal muscle
- signaling pathway
- smoking cessation
- deep learning
- cell cycle arrest