High fidelity one-pot DNA assembly using orthogonal serine integrases.

Jumai AbioyeMakeba Lawson-WilliamsAlicia LecandaBrecken CalhoonArlene L McQueSean D CollomsW Marshall StarkFemi J Olorunniji

Published in: Biotechnology journal (2022)

We conclude that use of multiple orthogonal integrases may be the most predictable, efficient, and programmable approach for SIRA and other in vitro applications.

Keyphrases

circulating tumor
cell free
single molecule
protein kinase
circulating tumor cells