Rapid detection of viable microbes with CTC and CFDA using a fibre fluorescence spectroscopy system.

The limit of detection (LoD) is too high in the dip-probe approach for analysis, however the development of an approach measuring the fluorescence of single cells may improve this limitation. The development of new bacteria-specific fluorogenic dyes may also address this limitation. The ability to differentiate bacteria using these dyes may add value to measurements made to enumerate bacteria using CTC and CFDA.