Polyurethane/Polylactide-Blend Films Doped with Zinc Ions for the Growth and Expansion of Human Olfactory Ensheathing Cells (OECs) and Adipose-Derived Mesenchymal Stromal Stem Cells (ASCs) for Regenerative Medicine Applications.
Krzysztof MaryczMonika MarędziakJakub GrzesiakDariusz SzarekAnna LisJadwiga LaskaPublished in: Polymers (2016)
Polymeric biomaterials based on polyurethane and polylactide blends are promising candidates for regenerative medicine applications as biocompatible, bioresorbable carriers. In current research we showed that 80/20 polyurethane/polylactide blends (PU/PLDL) with confirmed biological properties in vitro may be further improved by the addition of ZnO nanoparticles for the delivery of bioactive zinc oxide for cells. The PU/PLDL blends were doped with different concentrations of ZnO (0.001%, 0.01%, 0.05%) and undertaken for in vitro biological evaluation using human adipose stromal stem cells (ASCs) and olfactory ensheathing cells (OECs). The addition of 0.001% of ZnO to the biomaterials positively influenced the morphology, proliferation, and phenotype of cells cultured on the scaffolds. Moreover, the analysis of oxidative stress markers revealed that 0.001% of ZnO added to the material decreased the stress level in both cell lines. In addition, the levels of neural-specific genes were upregulated in OECs when cultured on sample 0.001 ZnO, while the apoptosis-related genes were downregulated in OECs and ASCs in the same group. Therefore, we showed that PU/PLDL blends doped with 0.001% of ZnO exert beneficial influence on ASCs and OECs in vitro and they may be considered for future applications in the field of regenerative medicine.
Keyphrases
- quantum dots
- induced apoptosis
- cell cycle arrest
- stem cells
- oxidative stress
- room temperature
- endothelial cells
- endoplasmic reticulum stress
- visible light
- cell death
- reduced graphene oxide
- tissue engineering
- signaling pathway
- bone marrow
- pi k akt
- type diabetes
- adipose tissue
- dna damage
- insulin resistance
- dna methylation
- ionic liquid
- drug delivery
- cell therapy
- mesenchymal stem cells
- cancer therapy
- highly efficient
- ischemia reperfusion injury
- diabetic rats
- induced pluripotent stem cells