Amino acid permease 3 (aap3) coding sequence as a target for Leishmania identification and diagnosis of leishmaniases using high resolution melting analysis.
Karl Erik MüllerRicardo Andrade ZampieriJuliana Ide AokiSandra Marcia MuxelAudun Helge NerlandLucile Maria Floeter-WinterPublished in: Parasites & vectors (2018)
HRM analysis using the aap3 coding sequence as target is a relatively cheap, fast and robust strategy to detect and discriminate Leishmania species from all the endemic regions worldwide. The target and method proved to be useful in clinical, field and experimental samples, thus it could be used as a tool in diagnosis as well as ecological and epidemiological studies.