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Spectrally-selective mid-IR laser-induced inactivation of pathogenic bacteria.

Victor KompanetsSvetlana ShelyginaEteri TolordavaSergey I KudryashovIrina SaraevaAleksey RupasovOlga BaitsaevaRoman KhmelnitskiiAndrey IoninYulia YushinaSergey ChekalinMichael Kovalev
Published in: Biomedical optics express (2021)
Micrometer-thick layers of Pseudomonas aeruginosa bacteria were prepared on fluorite substrates and scanned by focused mid-IR femtosecond laser radiation that was spectrally tuned to achieve the selective excitation of either the stretching C-H vibrations (3 μm), or stretching C = O, C-N vibrations (6 μm) of the amide groups in the bacteria. The enhanced biocidal efficiency of the latter selective excitation, compared to the more uniform 3-μm laser excitation, was demonstrated by performing viability assays of laser-treated bacterial layers. The bacterial inactivation by the 6-μm ultrashort laser pulses is attributed to dissociative denaturation of lipids and proteins in the cell membranes and intra-cell nucleic acids.
Keyphrases
  • pseudomonas aeruginosa
  • single cell
  • cell therapy
  • energy transfer
  • high speed
  • high throughput
  • escherichia coli
  • mesenchymal stem cells
  • radiation therapy
  • drug resistant
  • high resolution