Metabolic Profiling of Vasorelaxant Extract from Malvaviscus arboreus by LC/QTOF-MS.
Sergio Rodríguez-MoralesBlanca Ocampo-MedinaNancy Romero-CeronioCuauhtémoc Alvarado-SánchezMiguel Ángel Vilchis-ReyesLuis Fernando Roa de la FuenteRolffy Ortiz-AndradeOswaldo Ignacio Hernández-AbreuPublished in: Chemistry & biodiversity (2021)
We aimed to develop a standardized methodology to determine the metabolic profile of organic extracts from Malvaviscus arboreus Cav. (Malvaceae), a Mexican plant used in traditional medicine for the treatment of hypertension and other illnesses. Also, we determined the vasorelaxant activity of these extracts by ex vivo rat thoracic aorta assay. Organic extracts of stems and leaves were prepared by a comprehensive maceration process. The vasorelaxant activity was determined by measuring the relaxant capability of the extract to decrease a contraction induced by noradrenaline (0.1 μM). The hexane extract induced a significant vasorelaxant effect in a concentration- and endothelium-dependent manner. Secondary metabolites, such as polyunsaturated fatty acids, terpenes and one flavonoid, were annotated by liquid chromatography/quadrupole time-of-flight mass spectrometry (LC/QTOF-MS) in positive ion mode. This exploratory study allowed us to identify bioactive secondary metabolites from Malvaviscus arboreus, as well as identify potentially-new vasorelaxant molecules and scaffolds for drug discovery.
Keyphrases
- ms ms
- mass spectrometry
- liquid chromatography
- drug discovery
- oxidative stress
- simultaneous determination
- tandem mass spectrometry
- high resolution mass spectrometry
- blood pressure
- high performance liquid chromatography
- anti inflammatory
- gas chromatography
- diabetic rats
- multiple sclerosis
- high resolution
- high glucose
- aortic valve
- spinal cord
- nitric oxide
- replacement therapy
- tissue engineering
- smoking cessation
- drug induced
- smooth muscle
- pulmonary hypertension