Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application.

Jian ZhongZhenqing YeSamuel W LenzChad R ClarkAdil BharuchaGianrico FarrugiaKeith D RobertsonZhiguo ZhangTamas OrdogJeong-Heon Lee

Published in: BMC genomics (2017)

This study provides comparative data on commercial DNA purification reagents applied to nanogram-range immunopreciptated ChIP DNA and evidence for the importance of storage conditions of low nanogram-range purified DNA. We verified consistent high performance of a subset of the tested reagents. These results will facilitate the improvement of ChIP-seq methodology for low-input applications.

Keyphrases

circulating tumor
circulating tumor cells
cell free
single molecule
high throughput
single cell
nucleic acid
big data
machine learning
dna methylation
artificial intelligence