Login / Signup

Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed.

Florian J MayerFranz RatzingerRalf L J SchmidtGeorg GreinerOlfert LandtAlexander Am EndeVictor Max CormanNicole Perkmann-NageleThomas Watkins-RiedelDagmar PetermannKaroline AbadirJosef Zweimüller-MayerRobert Strassl
Published in: Virulence (2020)
Currently, testing for coronavirus is performed with time and personnel consuming PCR assays. The aim of this study was to evaluate the sensitivity, specificity and capacity of a fully automated, random access high-throughput real-time PCR-based diagnostic platform for the detection of SARS-CoV-2. The NeuMoDx N96 system displayed an equal or better detection rate for SARS-CoV-2 compared with the LightCycler 480II system and showed a specificity of 100%. The median PCR run time for all 28 PCR runs was 91 (IQR 84-97) minutes. The capacity of the NeuMoDx N96 could easily surpass the capacity of most currently used molecular test systems and significantly reduce the turn-around time.
Keyphrases
  • real time pcr
  • high throughput
  • sars cov
  • respiratory syndrome coronavirus
  • single cell
  • machine learning
  • deep learning
  • fluorescent probe