Nanopore sequencing reveals genomic map of CTX-M-type extended-spectrum β-lactamases carried by Escherichia coli strains isolated from blue mussels (Mytilus edulis) in Norway.
Didrik H GrevskottFrancisco Salvà-SerraEdward R B MooreNachiket Prakash MarathePublished in: BMC microbiology (2020)
This study confirms the efficacy of combining Nanopore long-read and Illumina short-read sequencing for determining complete bacterial genome sequences, enabling detection and characterization of clinically important ARGs in the marine environment in Norway, with potential for further dissemination. It also highlights the need for environmental surveillance of antibiotic resistance in low prevalence settings like Norway.
Keyphrases
- single molecule
- escherichia coli
- single cell
- klebsiella pneumoniae
- public health
- human health
- risk factors
- loop mediated isothermal amplification
- risk assessment
- gene expression
- dna methylation
- antibiotic resistance genes
- microbial community
- label free
- high density
- pseudomonas aeruginosa
- real time pcr
- sensitive detection