Metabolic differences in ripening of Solanum lycopersicum 'Ailsa Craig' and three monogenic mutants.
Stephan BeiskenMark EarllCharles BaxterDavid PortwoodZsuzsanna AmentAniko KendeCharlie HodgmanGraham SeymourRebecca SmithPaul FraserMark SeymourReza M SalekChristoph SteinbeckPublished in: Scientific data (2014)
Application of mass spectrometry enables the detection of metabolic differences between groups of related organisms. Differences in the metabolic fingerprints of wild-type Solanum lycopersicum and three monogenic mutants, ripening inhibitor (rin), non-ripening (nor) and Colourless non-ripening (Cnr), of tomato are captured with regard to ripening behaviour. A high-resolution tandem mass spectrometry system coupled to liquid chromatography produced a time series of the ripening behaviour at discrete intervals with a focus on changes post-anthesis. Internal standards and quality controls were used to ensure system stability. The raw data of the samples and reference compounds including study protocols have been deposited in the open metabolomics database MetaboLights via the metadata annotation tool Isatab to enable efficient re-use of the datasets, such as in metabolomics cross-study comparisons or data fusion exercises.
Keyphrases
- mass spectrometry
- liquid chromatography
- tandem mass spectrometry
- high resolution
- ultra high performance liquid chromatography
- high performance liquid chromatography
- gas chromatography
- wild type
- high resolution mass spectrometry
- simultaneous determination
- capillary electrophoresis
- solid phase extraction
- electronic health record
- emergency department
- rna seq
- single cell
- multidrug resistant
- gram negative
- label free
- quality improvement