Tandem Repeat Generation and Novel Isothermal Amplification Based on Nonspecific Tailing and Replication Slippage.

Guangcheng LuoHongfei HeDongsheng WangShanshan LiuShisu TianMiaomiao ChenQiang WangChangsong ZhaoZhengwei LengLingmi HouXiaolan Guo

Published in: Clinical chemistry (2023)

We discovered the mechanism for Bst-mediated nonspecific TRs generation and developed a novel isothermal amplification assay (BASIS), which can detect nucleic acids with high sensitivity and specificity.

Keyphrases

nucleic acid
high throughput
single cell