Chitosan/Gelatin Scaffolds Loaded with Jatropha mollissima Extract as Potential Skin Tissue Engineering Materials.
Matheus Ferreira de SouzaHenrique Nunes da SilvaJosé Filipe Bacalhau RodriguesMaria Dennise Medeiros MacêdoWladymyr Jefferson Bacalhau de SousaRossemberg Cardoso BarbosaMarcus Vinícius Lia FookPublished in: Polymers (2023)
This work aimed to develop chitosan/gelatin scaffolds loaded with ethanolic extract of Jatropha mollissima (EEJM) to evaluate the influence of its content on the properties of these structures. The scaffolds were prepared by freeze-drying, with different EEJM contents (0-10% ( w / w )) and crosslinked with genipin (0.5% ( w / w )). The EEJM were characterized through High Performance Liquid Chromatography coupled to a Diode Array Detector (HPLC-DAD), and the determination of three secondary metabolites contents was accomplished. The physical, chemical and biological properties of the scaffolds were investigated. From the HPLC-DAD, six main substances were evidenced, and from the quantification of the total concentration, the condensed tannins were the highest (431.68 ± 33.43 mg·g -1 ). Spectroscopy showed good mixing between the scaffolds' components. Adding and increasing the EEJM content did not significantly influence the properties of swelling and porosity, but did affect the biodegradation and average pore size. The enzymatic biodegradation test showed a maximum weight loss of 42.89 within 28 days and reinforced the efficiency of genipin in crosslinking chitosan-based materials. The addition of the extract promoted the average pore sizes at a range of 138.44-227.67 µm, which is compatible with those reported for skin regeneration. All of the scaffolds proved to be biocompatible for L929 cells, supporting their potential application as skin tissue engineering materials.
Keyphrases
- tissue engineering
- wound healing
- ms ms
- high performance liquid chromatography
- simultaneous determination
- drug delivery
- solid phase extraction
- tandem mass spectrometry
- weight loss
- mass spectrometry
- high resolution
- oxidative stress
- hyaluronic acid
- soft tissue
- induced apoptosis
- stem cells
- bariatric surgery
- cancer therapy
- type diabetes
- drug release
- magnetic resonance imaging
- computed tomography
- gastric bypass
- single molecule
- signaling pathway
- high density
- magnetic resonance
- nitric oxide