Inducible apelin receptor knockdown reduces differentiation efficiency and contractility of hESC-derived cardiomyocytes.

Robyn G C MacraeMaria T ColzaniThomas L WilliamsSemih BayraktarRhoda E KucAnna L PullingerWilliam G BernardEmma Louise RobinsonEmma E DavenportJanet J MaguireSanjay SinhaAnthony P Davenport

Published in: Cardiovascular research (2022)

We have successfully knocked down the apelin receptor, using an inducible system, to demonstrate a key role in hESC-CM differentiation. Knockdown in 3D engineered heart tissues recapitulated the phenotype of apelin receptor down-regulation in a failing heart, providing a potential platform for modelling heart failure and testing novel therapeutic strategies.

Keyphrases

heart failure
atrial fibrillation
gene expression
high throughput
left ventricular