Drosophila macrophages switch to aerobic glycolysis to mount effective antibacterial defense.
Gabriela KrejčováAdéla DanielováPavla NedbalováMichalina KazekLukáš StrychGeetanjali ChawlaJason M TennessenJaroslava LieskovskáMarek JindraTomáš DoležalAdam BajgarPublished in: eLife (2019)
Macrophage-mediated phagocytosis and cytokine production represent the front lines of resistance to bacterial invaders. A key feature of this pro-inflammatory response in mammals is the complex remodeling of cellular metabolism towards aerobic glycolysis. Although the function of bactericidal macrophages is highly conserved, the metabolic remodeling of insect macrophages remains poorly understood. Here, we used adults of the fruit fly Drosophila melanogaster to investigate the metabolic changes that occur in macrophages during the acute and resolution phases of Streptococcus-induced sepsis. Our studies revealed that orthologs of Hypoxia inducible factor 1α (HIF1α) and Lactate dehydrogenase (LDH) are required for macrophage activation, their bactericidal function, and resistance to infection, thus documenting the conservation of this cellular response between insects and mammals. Further, we show that macrophages employing aerobic glycolysis induce changes in systemic metabolism that are necessary to meet the biosynthetic and energetic demands of their function and resistance to bacterial infection.
Keyphrases
- drosophila melanogaster
- inflammatory response
- high intensity
- adipose tissue
- drug induced
- intensive care unit
- acute kidney injury
- liver failure
- transcription factor
- single molecule
- deep learning
- toll like receptor
- single cell
- escherichia coli
- candida albicans
- staphylococcus aureus
- lipopolysaccharide induced
- biofilm formation
- neural network