Potential of anaerobic digestate of dairy manure in suppressing soil-borne plant disease.
Zhifei PanGuangdou QiFetra J AndriamanohiarisoamananaTakaki YamashiroMasahiro IwasakiTakehiro NishidaSuchon TangtaweewipatKazutaka UmetsuPublished in: Animal science journal = Nihon chikusan Gakkaiho (2018)
Frequent use of pesticides to control soil-borne plant disease leads to environmental pollution and the development of pesticide resistance in phytopathogens. Soil amendment is considered to have the potential of suppressing plant disease because of its biological properties. However, information on anaerobic digestate is limited. In this study, potential of antagonistic activities of anaerobic digestate against phytopathogens were investigated by detecting the amounts of antagonistic bacteria (Bacillus and Pseudomonas) in anaerobic digestates of dairy manure. The results showed that anaerobic digestion increased the total amounts of Bacillus and Pseudomonas in digestate. Bacillus suppressed growth of phytopathogens, while Pseudomonas did not show any antagonistic activities. These results indicated that Bacillus was an effective antagonistic bacterium in digestate against phytopathogens. Furthermore, two selected isolates, B11 (Bacillus subtilis) and B59 (Bacillus licheniformis), were applied in field experiments and showed significant reduction in percent infection of potato late blight (Phytophthora infestans). These results demonstrate the benefits of digestate in suppressing soil-borne plant diseases caused by antagonistic bacteria.
Keyphrases
- sewage sludge
- anaerobic digestion
- bacillus subtilis
- plant growth
- heavy metals
- risk assessment
- antibiotic resistance genes
- human health
- municipal solid waste
- microbial community
- signaling pathway
- biofilm formation
- health risk assessment
- cell wall
- particulate matter
- healthcare
- high resolution
- health information
- social media
- staphylococcus aureus
- life cycle