Detection of tumor-derived extracellular vesicles in plasma from patients with solid cancer.
Silvia R VitaleJean A HelmijrMarjolein GerritsenHicret CobanLisanne F van DesselNick BeijeMichelle van der Vlugt-DaanePaolo VigneriAnieta M SieuwertsNatasja DitsMartin E van RoyenGuido JensterStefan SleijferMartijn LolkemaJohn W M MartensMaurice P H M JansenPublished in: BMC cancer (2021)
We provide a workflow for ctEV detection by spin column-based generic isolation of EVs and PCR-based measurement of gene expression and mutant transcripts in EV-RNA derived from cancer patients' blood plasma. This workflow, however, detected tumor-specific mutations in blood less often in EV-RNA than in cfDNA.