A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle.

Scott MartinAbhilash I ChiramelMarie Luisa SchmidtYu-Chi ChenNadia WhittAri WattEric C DunhamKyle ShifflettShelby TraegerAnne LeskeEugen BuehlerCynthia MartellaroJanine BrandtLisa WendtAndreas MüllerStephanie PeitschSonja M BestJürgen StechStefan FinkeAngela Römer-OberdörferAllison GrosethHeinz FeldmannThomas Hoenen

Published in: Genome medicine (2018)

This study provides a minable data set for every human gene regarding its role in EBOV genome replication and transcription, shows that an FDA-approved drug targeting one of the identified pathways is highly efficacious in vitro, and demonstrates the power of life cycle modelling systems for conducting genome-wide host factor screens for BSL4 viruses.

Keyphrases

genome wide
life cycle
dna methylation
copy number
endothelial cells
cancer therapy
gene expression
emergency department
induced pluripotent stem cells
adverse drug