Identification of new DNA-associated proteins from Waddlia chondrophila.
Marie de BarsyLucas HerrgottVirginie MartinTrestan PillonelPatrick H ViollierGilbert GreubPublished in: Scientific reports (2019)
Transcriptional regulation in Chlamydiae is still poorly understood. The absence until recently of genetic tools is the main cause of this gap. We discovered three new potential DNA-associated proteins of Waddlia chondrophila, a Chlamydia-related bacterium, using heparin chromatography coupled to mass spectrometry (Wcw_0377, Wcw_1456, and Wcw_1460). By ChIP-seq analysis, we determined the regulatory landscape of these three proteins and we showed that Wcw_0377 binds all along the genome whereas Wcw_1456 and _1460 possess a wide regulon with a large number of co-regulated genes. Wcw_1456 and Wcw_1460 interact with RpoD (σ66), emerging as potential RpoD regulators. On the other hand, Wcw_0377 is able to reach the host nucleus, where it might interact with eukaryotic histones through its putative chromatin-remodelling SWIB/MDM2 domain.
Keyphrases
- genome wide
- mass spectrometry
- transcription factor
- circulating tumor
- liquid chromatography
- dna methylation
- single molecule
- cell free
- single cell
- high performance liquid chromatography
- gene expression
- bioinformatics analysis
- circulating tumor cells
- dna damage
- copy number
- high speed
- venous thromboembolism
- human health
- gas chromatography
- genome wide identification
- tandem mass spectrometry
- rna seq