A high throughput immuno-affinity mass spectrometry method for detection and quantitation of SARS-CoV-2 nucleoprotein in human saliva and its comparison with RT-PCR, RT-LAMP, and lateral flow rapid antigen test.
Dan LaneRebecca AllsoppChristopher W HolmesOliver C SlingsbyRebekah Jukes-JonesPaul BirdN Leigh AndersonMorteza RazaviRichard YipTerry W PearsonMatt PopeKamlesh KhuntiIvan DoykovJenny HällqvistKevin MillsPaul SkippRachel S CarlingLeong NgJacqui ShawPankaj GuptaDonald J L JonesPublished in: Clinical chemistry and laboratory medicine (2024)
Detection of SARS-CoV-2 nucleoprotein in saliva was less frequent than the detection of viral RNA. The SISCAPA-LC-MS method allowed processing of multiple samples in <150 min and was scalable, enabling high throughput.
Keyphrases
- loop mediated isothermal amplification
- sars cov
- high throughput
- mass spectrometry
- real time pcr
- sensitive detection
- respiratory syndrome coronavirus
- label free
- liquid chromatography
- endothelial cells
- high resolution
- ms ms
- tandem mass spectrometry
- gas chromatography
- quantum dots
- coronavirus disease
- solid phase extraction