We present a versatile extended depth-of-field (EDOF) wide-field fluorescence microscopy using a new, to the best of our knowledge, active device, micro-mirror array lens system (MALS) for calibration-free and orientation-insensitive EDOF imaging. The MALS changed the focal plane during image acquisition, and the system could be operated in any orientation. Two EDOF imaging modes of high-speed accumulation and low-speed surface sectioning were implemented. The performance was demonstrated in non-contact imaging of conjunctival goblet cells in live mice and depth-resolved cellular examination of ex-vivo human cancer specimens. MALS-based EDOF microscopy has potential for versatile cellular examination.
Keyphrases
- high resolution
- high speed
- single molecule
- optical coherence tomography
- atomic force microscopy
- high throughput
- mass spectrometry
- induced apoptosis
- endothelial cells
- cell death
- squamous cell carcinoma
- papillary thyroid
- deep learning
- young adults
- label free
- fluorescence imaging
- insulin resistance
- high fat diet induced
- endoplasmic reticulum stress
- adipose tissue
- human health
- high density