Detection of Leptin Using Electrocatalyst Mediated Impedimetric Sensing.
Tamanna IslamMd Ariful AhsanMasud HassanHumayra AfrinJaqueline Pena-ZacariasAli K AldalbahiBonifacio Alvarado-TenorioJuan C NoveronMd NurunnabiPublished in: ACS biomaterials science & engineering (2022)
Obesity is a complex disorder associated with immense health consequences including high risk of cardiovascular diseases, diabetes, and cancer. Abnormality in the thyroid gland, genetics, less physical activity, uptake of excessive diet, and leptin resistance are critical factors in the development of obesity. To determine the treatment strategy, understanding the pathophysiology of obesity is crucial. For instances, leptin resistance mediated obesity defined by the presence of excessive leptin hormone (Lep) in the systemic circulation is very common in diet induced obesity. Therefore, our hypothesis is that quantitative measurement of Lep from blood can help to identify individuals with Lep resistant mediated obesity and thereby guide toward a proper treatment strategy. In this work, we aim to utilize an electrochemical immunosensing platform for diagnosis of obesity by measuring the Lep content in systemic circulation. A porous carbon confined FeNi bimetallic system was synthesized with three different ratios of Fe and Ni ions using high temperature pyrolysis technique. The suitability of the sensor for detecting Lep was studied using both CV and EIS techniques. The limit of detection (LOD) for GCE was recorded as 157.4 fg/mL with a wide linear concentration range of 500 fg/mL to 80 ng/mL, while for SPCE the LOD was 184.9 fg/mL with a linear range of 500 fg/mL to 50 ng/mL. Finally, the feasibility and applicability of the sensor for Lep detection was tested with serum collected from high fat diet induced obese rats. The selectivity, sensitivity, storage, and experimental stability and reproducibility tests showed potential for this biosensor platform as a point-of-care Lep detection device.
Keyphrases
- high fat diet induced
- weight loss
- insulin resistance
- metabolic syndrome
- weight gain
- type diabetes
- label free
- physical activity
- bariatric surgery
- adipose tissue
- cardiovascular disease
- healthcare
- public health
- body mass index
- gold nanoparticles
- loop mediated isothermal amplification
- mental health
- high throughput
- cardiovascular events
- glycemic control
- metal organic framework
- coronary artery disease
- social media
- sleep quality