Mapping three-dimensional intratumor proteomic heterogeneity in uterine serous carcinoma by multiregion microsampling.

Allison L HuntNicholas W BatemanWaleed BarakatSasha C Makohon-MooreTamara AbulezJordan A DriscollJoshua P SchaafBrian L HoodKelly A ConradsMing ZhouValerie CalvertMariaelena PierobonJeremy LoffredoKatlin N WilsonTracy J LitziPang-Ning TengJulie OliverDave MitchellGlenn GistChristine RojasBrian BlantonKathleen M DarcyUma N M RaoEmanuel F PetricoinNeil T PhippenG Larry MaxwellThomas P Conrads

Published in: Clinical proteomics (2024)

Here we identified extensive three-dimensional heterogeneity within the USC tumor microenvironment, with disease-relevant biomarkers present in both the tumor and the stroma. These data underscore the critical need for upfront enrichment of cellular subpopulations from tissue specimens for spatial proteogenomic analysis.

Keyphrases

single cell
high grade
high resolution
electronic health record
big data
machine learning
fine needle aspiration