Interference of Antinuclear Antibody (ANA) in Indirect Immunofluorescence Assay (IIFA)-Based Perinuclear Antineutrophil Cytoplasmic Antibody (pANCA) Interpretation.

Sangeeta Deka Deepjyoti Kalita Udaykumar Sasi Rekha Mahanta Putul Diksha Rani Ravi Shankar Anusha Krishna Raj Mithilesh Kumar Jha Gaurav Badoni Manisha Paul Shailesh Kumar Gupta Shailender Negi Anshu Singh Kuhu Chatterjee

Published in: Autoimmune diseases (2022)

ANA-positive results may interfere with the interpretation of pANCA as observed in ANA-IIFA and ENA-blot positive samples. ANA-IIFA patterns like nuclear homogenous may strongly associate this pANCA interpretation. This can help laboratories perform ANCA testing more effectively, ruling out ANA interference in ANCA screening.

Keyphrases

high throughput
single cell