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Effect of MnO 2 Nanoparticles Stabilized with Cocamidopropyl Betaine on Germination and Development of Pea ( Pisum sativum L.) Seedlings.

Andrey Ashotovich NagdalianAndrey BlinovAlexey GvozdenkoAlexey GolikZafar A RekhmanIgor Vladimirovich RzhepakovskyRoman KolesnikovSvetlana AvanesyanAnastasiya BlinovaMaxim A PirogovPavel LeontevAlina AskerovaEvgeniy TsykinMohammad Ali Shariati
Published in: Nanomaterials (Basel, Switzerland) (2024)
This study aimed to synthesize, characterize, and evaluate the effect of cocamidopropyl betaine-stabilized MnO 2 nanoparticles (NPs) on the germination and development of pea seedlings. The synthesized NPs manifested as aggregates ranging from 50-600 nm, comprising spherical particles sized between 19 to 50 nm. These particles exhibited partial crystallization, indicated by peaks at 2θ = 25.37, 37.62, 41.18, 49.41, 61.45, and 65.79°, characteristic of MnO 2 with a tetragonal crystal lattice with a I4/m spatial group. Quantum chemical modelling showed that the stabilization process of MnO 2 NPs with cocamidopropyl betaine is energetically advantageous (∆E > 1299.000 kcal/mol) and chemically stable, as confirmed by the positive chemical hardness values (0.023 ≤ η ≤ 0.053 eV). It was revealed that the interaction between the MnO 2 molecule and cocamidopropyl betaine, facilitated by a secondary amino group (NH), is the most probable scenario. This ascertain is supported by the values of the difference in total energy (∆E = 1299.519 kcal/mol) and chemical hardness (η = 0.053 eV). These findings were further confirmed using FTIR spectroscopy. The effect of MnO 2 NPs at various concentrations on the germination of pea seeds was found to be nonlinear and ambiguous. The investigation revealed that MnO 2 NPs at a concentration of 0.1 mg/L resulted in the highest germination energy (91.25%), germinability (95.60%), and lengths of roots and seedlings among all experimental samples. However, an increase in the concentration of preparation led to a slight growth suppression (1-10 mg/L) and the pronounced inhibition of seedling and root development (100 mg/L). The analysis of antioxidant indicators and phytochemicals in pea seedlings indicated that only 100 mg/L MnO 2 NPs have a negative effect on the content of soluble sugars, chlorophyll a/b, carotenoids, and phenols. Conversely, lower concentrations showed a stimulating effect on photosynthesis indicators. Nevertheless, MnO 2 NPs at all concentrations generally decreased the antioxidant potential of pea seedlings, except for the ABTS parameter. Pea seedlings showed a notable capacity to absorb Mn, reaching levels of 586.5 μg/L at 10 mg/L and 892.6 μg/L at 100 mg/L MnO 2 NPs, surpassing the toxic level for peas according to scientific literature. However, the most important result was the observed growth-stimulating activity at 0.1 mg/L MnO 2 NPs stabilized with cocamidopropyl betaine, suggesting a promising avenue for further research.
Keyphrases
  • arabidopsis thaliana
  • oxide nanoparticles
  • oxidative stress
  • systematic review
  • single cell
  • single molecule
  • climate change
  • metal organic framework