Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method.
Jakob BlomgrenFredrik AhrentorpDag IlverChristian JonassonSobhan SepehriAlexei KalaboukhovDag WinklerTeresa Zardán Gómez de la TorreMaria StrømmeChrister JohanssonPublished in: Nanomaterials (Basel, Switzerland) (2018)
We developed a novel biodetection method for influenza virus based on AC magnetic susceptibility measurement techniques (the DynoMag induction technique) together with functionalized multi-core magnetic nanoparticles. The sample consisting of an incubated mixture of magnetic nanoparticles and rolling circle amplified DNA coils is injected into a tube by a peristaltic pump. The sample is moved as a plug to the two well-balanced detection coils and the dynamic magnetic moment in each position is read over a range of excitation frequencies. The time for making a complete frequency sweep over the relaxation peak is about 5 minutes (10 Hz⁻10 kHz with 20 data points). The obtained standard deviation of the magnetic signal at the relaxation frequency (around 100 Hz) is equal to about 10-5 (volume susceptibility SI units), which is in the same range obtained with the DynoMag system. The limit of detection with this method is found to be in the range of 1 pM.
Keyphrases
- magnetic nanoparticles
- molecularly imprinted
- single molecule
- air pollution
- loop mediated isothermal amplification
- label free
- real time pcr
- particulate matter
- high frequency
- electronic health record
- circulating tumor
- machine learning
- big data
- high resolution
- risk assessment
- mass spectrometry
- artificial intelligence
- liquid chromatography
- ionic liquid