Tissue Engineering to Repair Diaphragmatic Defect in a Rat Model.
G P LiaoYohan ChoiK VojnitsH XueK AroomF MengH Y PanR A HetzC J CorkinsT G HughesF TrioloA JohnsonKenneth J MoiseK P LallyCharles S CoxYong LiPublished in: Stem cells international (2017)
Tissue engineering is an emerging strategy for repairing damaged tissues or organs. The current study explored using decellularized rat diaphragm scaffolds combined with human amniotic fluid-derived multipotent stromal cells (hAFMSC) to provide a scaffold, stem cell construct that would allow structural barrier function during tissue ingrowth/regeneration. We created an innovative cell infusion system that allowed hAFMSC to embed into scaffolds and then implanted the composite tissues into rats with surgically created left-sided diaphragmatic defects. Control rats received decellularized diaphragm scaffolds alone. We found that the composite tissues that combined hAFMSCs demonstrated improved physiological function as well as the muscular-tendon structure, compared with the native contralateral hemidiaphragm of the same rat. Our results indicate that the decellularized diaphragm scaffolds are a potential support material for diaphragmatic hernia repair and the composite grafts with hAFMSC are able to accelerate the functional recovery of diaphragmatic hernia.
Keyphrases
- tissue engineering
- stem cells
- mechanical ventilation
- gene expression
- endothelial cells
- oxidative stress
- low dose
- cell therapy
- single cell
- intensive care unit
- risk assessment
- body composition
- resistance training
- human health
- bone marrow
- acute respiratory distress syndrome
- rotator cuff
- anterior cruciate ligament reconstruction