Hyperthermia and doxorubicin release by Fol-LSMO nanoparticles induce apoptosis and autophagy in breast cancer cells.
Neha Kulkarni-DwivediPratikshkumar R PatelBhupendra V ShravageRinku D UmraniKishore M PaknikarSachin Hanmant JadhavPublished in: Nanomedicine (London, England) (2023)
Background: Studies on the anticancer effects of lanthanum strontium manganese oxide (LSMO) nanoparticles (NPs)-mediated hyperthermia at cellular and molecular levels are scarce. Materials & methods: LSMO NPs conjugated with folic acid (Fol-LSMO NPs) were synthesized, followed by doxorubicin-loading (DoxFol-LSMO NPs), and their effects on breast cancer cells were investigated. Results: Hyperthermia (45°C) and combination treatments exhibited the highest (∼95%) anticancer activity with increased oxidative stress. The involvement of intrinsic mitochondria-mediated apoptotic pathway and induction of autophagy was noted. Cellular and molecular evidence confirmed the crosstalk between apoptosis and autophagy, involving Beclin1 , Bcl2 and Caspase-3 genes with free reactive oxygen species presence. Conclusion: The study confirmed hyperthermia and doxorubicin release by Fol-LSMO NPs induces apoptosis and autophagy in breast cancer cells.
Keyphrases
- cell death
- breast cancer cells
- oxidative stress
- endoplasmic reticulum stress
- oxide nanoparticles
- cell cycle arrest
- induced apoptosis
- reactive oxygen species
- drug delivery
- cancer therapy
- signaling pathway
- ischemia reperfusion injury
- dna damage
- photodynamic therapy
- single molecule
- mass spectrometry
- genome wide identification
- walled carbon nanotubes
- genome wide analysis