Enzyme intermediates captured "on the fly" by mix-and-inject serial crystallography.
Jose L OlmosSuraj PandeyJose M Martin-GarciaGeorge CalveyAndrea KatzJuraj KnoskaChristopher KupitzMark S HunterMengning LiangDominik OberthuerOleksandr YefanovMax WiedornMichael HeymanMark HollKanupriya PandeAnton BartyMitchell D MillerStephan SternShatabdi Roy-ChowdhuryJesse CoeNirupa NagaratnamJames ZookJacob VerburgtTyler NorwoodIshwor PoudyalDavid XuJason KoglinMatthew H SeabergYun ZhaoSaša BajtThomas GrantValerio MarianiGarrett NelsonGanesh SubramanianEuiyoung BaeRaimund FrommeRussell FungPeter SchwanderMatthias FrankThomas A WhiteUwe WeierstallNadia ZatsepinJohn SpencePetra FrommeHenry N ChapmanLois PollackLee TremblayAbbas OurmazdGeorge N PhillipsMarius SchmidtPublished in: BMC biology (2018)
MISC is a versatile and generally applicable method to investigate reactions of biological macromolecules, some of which are of immense biological significance and might be, in addition, important targets for structure-based drug design. With megahertz X-ray pulse rates expected at the Linac Coherent Light Source II and the European X-ray free-electron laser, multiple, finely spaced time delays can be collected rapidly, allowing a comprehensive description of biomolecular reactions in terms of structure and kinetics from the same set of X-ray data.