Role of the Transient Receptor Potential Vanilloid Type 1 (TRPV1) in the Regulation of Nitric Oxide Release in Wistar Rat Aorta.
Elvira Varela LópezLeonardo Del Valle-MondragonVicente Castrejón-TéllezIsrael Pérez-TorresAraceli Páez ArenasFelipe Massó RojasVerónica Guarner-LansAlvaro Vargas-GonzálezGustavo Pastelín-HernándezJuan Carlos Torres-NarváezPublished in: Oxidative medicine and cellular longevity (2021)
The potential transient vanilloid receptor type 1 (TRPV1) plays important functional roles in the vascular system. In the present study, we explored the role of the TRPV1 in the production of nitric oxide (NO), biopterines (BH4 and BH2), cyclic guanosine monophosphate (cGMP), malondialdehyde (MDA), phosphodiesterase-3 (PDE-3), total antioxidant capacity (TAC), and calcitonin gene-related peptide (CGRP) in the rat aorta. Wistar rats were divided into four groups: (1) control, (2) capsaicin (CS, 20 mg/kg), (3) capsazepine (CZ, 24 mg/kg), and (4) CZ + CS. Treatments were applied daily for 4 days before removing the thoracic aortas for testing of aortic tissue and endothelial cells. TRPV1 activation produced increases in BH4 14%, cGMP 25%, NO 29%, and TAC 59.2% in comparison to the controls. BH2 and MDA increased with CZ. CGRP shows a tendency to decrease with CZ. The analysis by immunocytochemistry confirmed that the TRPV1 is present in aortic endothelial cells. Aortic endothelial cells were obtained from healthy rats and cultured to directly explore the effects of CS and CZ. The activation of the TRPV1 (CS 30 μM) produced increases in BH4 17%, NO 36.6%, TAC 56.3%, and CGRP 65%, when compared to controls. BH2 decreased with CZ + CS. CS effects were diminished by CZ in cells and in the tissue. We conclude that the TRPV1 is a structure present in the membrane of aortic endothelial cells and that it participates in the production of NO. The importance of the TRPV1 should be considered in vascular reactivity studies.
Keyphrases
- endothelial cells
- nitric oxide
- aortic valve
- neuropathic pain
- pulmonary artery
- aortic dissection
- left ventricular
- high glucose
- spinal cord
- induced apoptosis
- cell cycle arrest
- oxidative stress
- pulmonary hypertension
- vascular endothelial growth factor
- nitric oxide synthase
- heart failure
- breast cancer cells
- copy number
- spinal cord injury
- risk assessment
- human health
- cell death
- signaling pathway
- endoplasmic reticulum stress
- clinical evaluation