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Nm-seq maps 2'-O-methylation sites in human mRNA with base precision.

Qing DaiSharon Moshitch-MoshkovitzDali HanNitzan KolNinette AmariglioGideon RechaviDan DominissiniChuan He
Published in: Nature methods (2017)
The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.
Keyphrases
  • photodynamic therapy
  • genome wide
  • single cell
  • rna seq
  • endothelial cells
  • high throughput
  • high resolution
  • light emitting
  • dna methylation
  • induced pluripotent stem cells
  • binding protein
  • mass spectrometry
  • high density