Long-Term Accurate Continuous Glucose Biosensors via Extended Nitric Oxide Release.
Maggie J Malone-PovolnyElizabeth P MerricksLauren E WimseyTimothy C NicholsMark H SchoenfischPublished in: ACS sensors (2019)
Analytical performance and tissue interactions of nitric oxide (NO)-releasing continuous glucose sensors were evaluated over a 28 d study in a diabetic swine model. Interstitial glucose was detected using an implanted needle-type amperometric glucose sensor. Two NO-release durations from the sensor surface were achieved by doping the membranes with nonporous (14 d release) or porous (30 d release) S-nitrosothiol-functionalized silica nanoparticles. Numerical and clinical accuracy of the sensors were assessed at time points (1, 7, 14, 21, and 28 d) following implantation. Nitric oxide-releasing sensors demonstrated accurate glucose detection over a time period directly correlated with the active release of NO. Silica particle-doped sensors that released NO for 30 d showed standard-compliant accuracy (i.e., mean absolute relative difference ≤ 15%) for >3 weeks post-implantation. Histological staining for inflammatory biomarkers suggested that the observed performance improvement was the result of decreased inflammatory cell count and a lower density collagen capsule.
Keyphrases
- nitric oxide
- blood glucose
- hydrogen peroxide
- low cost
- nitric oxide synthase
- oxidative stress
- type diabetes
- quantum dots
- wound healing
- single cell
- ultrasound guided
- mass spectrometry
- gold nanoparticles
- mesenchymal stem cells
- weight loss
- skeletal muscle
- liquid chromatography
- sensitive detection
- loop mediated isothermal amplification
- molecularly imprinted