Detection of SARS-CoV-2 spike protein D614G mutation using μTGGE.
Kevin Maafu JumaKenta MorimotoVishnu SharmaKirti SharmaRadhika BiyaniManish BiyaniTeisuke TakitaKiyoshi YasukawaPublished in: Molecular biology reports (2024)
Our results demonstrate the capability of μTGGE for the rapid, precise, and cost-effective detection of SARS-CoV-2 spike protein D614 and G614 variants without the need for sequencing. Therefore, this approach holds considerable potential for use in point-of-care mutation assays for SARS-CoV-2 and other pathogens.
Keyphrases
- sars cov
- loop mediated isothermal amplification
- respiratory syndrome coronavirus
- protein protein
- label free
- real time pcr
- binding protein
- amino acid
- high throughput
- single cell
- copy number
- small molecule
- gene expression
- dna methylation
- multidrug resistant
- coronavirus disease
- risk assessment
- high throughput sequencing