A new method for functional analysis of plastid EMBRYO-DEFECTIVE PPR genes by efficiently constructing cosuppression lines in Arabidopsis.

Jingli ChenHaojie ZhuJirong HuangWeihua Huang

Published in: Plant methods (2020)

Overexpression of an appropriately truncated EMB PPR CDS in WT leads to gene silencing in a RDR6-dependent manner, and this method can be employed to study the unknown function of EMB PPR genes. By this method, we showed that EMB976 is required for splicing of chloroplast clpP1 intron 2 and ycf3 intron 1.

Keyphrases

transcription factor
genome wide
genome wide identification
bioinformatics analysis
cell proliferation
genome wide analysis
dna methylation
gene expression
pregnant women
pregnancy outcomes