Background and Purpose: An absence of specific and precise instruments that measure the construct of intrapersonal and interpersonal communication in nursing was verified. This study aimed to construct and validate the intrapersonal and interpersonal communication scale in nursing. Methods: A psychometric study was conducted on a sample of 360 nurses from a hospital and university center in the central region of Portugal. Reliability was assessed through internal consistency and construct validity through exploratory and confirmatory factor analyses. Results: Four factors were decided upon: "self-efficacy," "empathy," "assertiveness," and "self-knowledge." Good internal consistency was obtained with Cronbach's alpha and adequate quality of adjustment indexes for the model. Conclusions: The key global indicators of the model's adjustment and reliability analysis express their quality for the Portuguese population.