In vivo metal-catalyzed SeCT therapy by a proapoptotic peptide.
Peni AhmadiKyohei MugurumaTsung-Che ChangSatoru TamuraKazuki TsubokuraYasuko EgawaTakehiro SuzukiNaoshi DohmaeYoichi NakaoKatsunori TanakaPublished in: Chemical science (2021)
Selective cell tagging (SeCT) therapy is a strategy for labeling a targeted cell with certain chemical moieties via a catalytic chemical transformation in order to elicit a therapeutic effect. Herein, we report a cancer therapy based on targeted cell surface tagging with proapoptotic peptides (Ac-GGKLFG-X; X = reactive group) that induce apoptosis when attached to the cell surface. Using either Au-catalyzed amidation or Ru-catalyzed alkylation, these proapoptotic peptides showed excellent therapeutic effects both in vitro and in vivo. In particular, co-treatment with proapoptotic peptide and the carrier-Ru complex significantly and synergistically inhibited tumor growth and prolonged survival rate of tumor-bearing mice after only a single injection. This is the first report of Ru catalyst application in vivo, and this approach could be used in SeCT for cancer therapy.
Keyphrases
- cancer therapy
- cell surface
- room temperature
- drug delivery
- single cell
- cell therapy
- energy transfer
- oxidative stress
- endoplasmic reticulum stress
- type diabetes
- amino acid
- mesenchymal stem cells
- highly efficient
- bone marrow
- metabolic syndrome
- gold nanoparticles
- skeletal muscle
- insulin resistance
- crystal structure
- chemotherapy induced