Murine Gbp1 and Gbp2 are ubiquitinated independent of Toxoplasma gondii infection.
Vesela EnchevaClémence FoltzAmbrosius P SnijdersEva-Maria FrickelPublished in: BMC research notes (2018)
We infected IFNγ-stimulated murine embryonic fibroblasts (MEFs) from either C57BL/6×129 wild-type (WT) mice or C57BL/6 TRIM21-/- mice with Toxoplasma. Using mass spectrometry, we analysed proteins in both cell backgrounds presenting with the di-glycine remnant of ubiquitination. In addition, we compared peptide levels between WT and TRIM21-/- cells. In line with earlier reports, Gbp1 was expressed to higher levels in the C57BL/6×129 WT MEFs compared to the C57BL/6-only background TRIM21-/- MEFs. Protein expression differences in these different murine backgrounds thus precluded identification of TRIM21-dependent ubiquitinated substrates. Nevertheless, we identified and confirmed Gbp1 and Gbp2 as being ubiquitinated in a Toxoplasma-infection independent manner.
Keyphrases
- wild type
- toxoplasma gondii
- mass spectrometry
- induced apoptosis
- high fat diet induced
- immune response
- single cell
- dendritic cells
- type diabetes
- emergency department
- liquid chromatography
- biofilm formation
- extracellular matrix
- staphylococcus aureus
- high performance liquid chromatography
- case report
- signaling pathway
- cell therapy
- cystic fibrosis
- insulin resistance
- adipose tissue
- gas chromatography
- ms ms
- oxidative stress
- capillary electrophoresis
- tandem mass spectrometry