Label-free immunosensing of telomerase using bio-conjugation of biotinylated antibody to poly(chitosan) gold nanoparticles.

Aim: This study aimed to establish a label-free electrochemical biosensor for telomerase detection in human biofluid. Method: Synthesized green nanocomposite (poly[chitosan] decorated by gold nanoparticles) was used for the efficient immobilization of biotinylated antibody of telomerase and immunocomplex of antigen-antibody. Poly(chitosan) was decorated by gold nanoparticles on the surface of a glassy carbon electrode using an electrochemical coating technique. Results: The constructed immunosensor exhibited wide dynamic range (0.078-160 IU/ml -1 ) with a low limit of quantification of 0.078 IU/ml -1 , which present a unique manner for telomerase assays in early prognosis for cancers. Conclusion: This study encourages scientists and scholars to design and develop new biosensor platforms for point-of-care diagnostics for telomerase management, an interesting reference for future research.