Ionic liquid modification reshapes the substrate pockets of lipase to boost its stability and activity in Vitamin E succinate synthesis.

In this study, a high-efficiency VES synthesis strategy was successfully developed via modification of lipase. Moreover, the mechanism in which the ILs modification can enhance the activity and stability of lipase was investigated via both experimental and computational-aided methods. Molecular dynamics simulation suggested that ILs modification changed the geometry of Phe344 from flat to upright, which significantly reshaped and enhanced the size of substrate binding pocket of CRL. It is also agreement with our circular dichroism (CD) and fluorescence spectroscopy results which suggested that the modification changed the secondary structure of CRL to a certain extent. The larger pocket also endowed the suitable binding pose of succinate, which made the hydrogen bonds between succinate and active site Ser209 become stronger, and thus improving the yield of VES. This article is protected by copyright. All rights reserved.