DNA Origami Inside-Out Viruses.
Jonathan R BurnsBaptiste LamarreAlice L B PyneJames E NobleMaxim G RyadnovPublished in: ACS synthetic biology (2018)
A synthetic topology for everted viruses is reported. The topology is a single-stranded virion DNA assembled into a hollow cube with exterior decorated with HIV-Tat transduction domains. The cube incorporates a pH-responsive lid allowing for the controlled encapsulation of functional proteins and their transfer and release into live cells. Unlike viruses, which are protein shells with a [3,5]-fold rotational symmetry that encase nucleic acids, these cubes are [3, 4]-fold DNA boxes encapsulating proteins. Like viruses, such everted DNA-built viruses are monodisperse nanoscale assemblies that infect human cells with a specialist cargo. The design offers a bespoke bottom-up platform for engineering nonpolyhedral, nonprotein synthetic viruses.
Keyphrases
- circulating tumor
- cell free
- nucleic acid
- induced apoptosis
- hiv infected
- high throughput
- hiv positive
- circulating tumor cells
- mass spectrometry
- cell death
- endoplasmic reticulum stress
- cell proliferation
- signaling pathway
- binding protein
- highly efficient
- hiv testing
- cell cycle arrest
- men who have sex with men
- atomic force microscopy