Acute microglia ablation induces neurodegeneration in the somatosensory system.
Stephen J RubinoLior MayoIsabella WimmerVictoria SiedlerFlorian BrunnerSimon HametnerAsaf MadiAmanda LanserThais MoreiraDustin DonnellyLaura CoxRafael Machado RezendeOleg ButovskyHans LassmannHoward L WeinerPublished in: Nature communications (2018)
Previous studies have reported that microglia depletion leads to impairment of synapse formation and these cells rapidly repopulate from CNS progenitors. However, the impact of microglia depletion and repopulation in the long-term state of the CNS environment has not been characterized. Here, we report that acute and synchronous microglia depletion and subsequent repopulation induces gray matter microgliosis, neuronal death in the somatosensory cortex and ataxia-like behavior. We find a type 1 interferon inflammatory signature in degenerating somatosensory cortex from microglia-depleted mice. Transcriptomic and mass cytometry analysis of repopulated microglia demonstrates an interferon regulatory factor 7-driven activation state. Minocycline and anti-IFNAR1 antibody treatment attenuate the CNS type 1 interferon-driven inflammation, restore microglia homeostasis and reduce ataxic behavior. Neither microglia depletion nor repopulation impact neuropathology or T-cell responses during experimental autoimmune encephalomyelitis. Together, we found that acute microglia ablation induces a type 1 interferon activation state of gray matter microglia associated with acute neurodegeneration.
Keyphrases
- inflammatory response
- neuropathic pain
- liver failure
- respiratory failure
- dendritic cells
- oxidative stress
- drug induced
- blood brain barrier
- single cell
- spinal cord injury
- aortic dissection
- spinal cord
- hepatitis b virus
- transcription factor
- intensive care unit
- immune response
- rna seq
- cell cycle arrest
- endoplasmic reticulum stress
- extracorporeal membrane oxygenation