Quercetin Prevents LPS-Induced Oxidative Stress and Inflammation by Modulating NOX2/ROS/NF-kB in Lung Epithelial Cells.
Ok-Joo SulSeung Won RaPublished in: Molecules (Basel, Switzerland) (2021)
Oxidative stress caused by the production of reactive oxygen species (ROS) plays a major role in inflammatory processes. We hypothesized that modulation of ROS via quercetin may protect against oxidative stress and inflammation. Thus, this study aimed to investigate the effects of quercetin on oxidative stress and inflammation in lung epithelial A549 cells. The lipopolysaccharide (LPS)-induced elevation of intracellular ROS levels was reduced after quercetin treatment, which also almost completely abolished the mRNA and protein expression of nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2) induced by LPS stimulation. In addition, quercetin suppressed the nuclear translocation of nuclear factor kappa B (NF-κB) and reduced levels of inflammatory cytokine tumor necrosis factor (TNF)-α, interleukin (IL)-1, and IL-6, which had increased significantly after LPS exposure. Our data demonstrated that quercetin decreased ROS-induced oxidative stress and inflammation by suppressing NOX2 production.
Keyphrases
- oxidative stress
- reactive oxygen species
- lps induced
- inflammatory response
- nuclear factor
- induced apoptosis
- dna damage
- toll like receptor
- diabetic rats
- ischemia reperfusion injury
- cell death
- rheumatoid arthritis
- signaling pathway
- cell cycle arrest
- anti inflammatory
- cell proliferation
- hydrogen peroxide
- nitric oxide
- immune response
- big data
- combination therapy
- heat shock protein
- mouse model