Efficient Capture and Traceless Release of Functional CD8 + T Cells with a Microfluidic Chip for Enhanced In Vitro and In Viv o CD4-CAR Transduction.
Aynur AbdullaTuersunayi AbudurehemanKai-Ming ChenBehafarid GhalandariHaoni YanHang ZhouHengxing SuYunqian ZhangCai-Wen DuanXianting DingPublished in: Analytical chemistry (2024)
The chimeric antigen receptor (CAR) T cells targeting CD4 expressed cells in acute lymphoblastic leukemia (T-ALL) and acute myeloid leukemia (AML) could reduce the risk of off target effects in normal tissues. However, the efficacy of adoptive cell therapy is predominantly attributed to CD8 + T cells, necessitating their purification before lentivirus transfection to enhance the production of CD4-CAR-T cells. In this study, we developed a microfluidic chip functionalized with an optimized CD8 aptamer, A3t-MU, to facilitate the enrichment and purification of CD8 + T cells. The presented chip showed efficient capture and seamless release of CD8 + T cells from cultured T cells and peripheral blood mononuclear cells (PBMCs). The purity of the released CD8 + T cells reached 98.1%, representing a 13% improvement over the conventional magnetic bead separation method. CD4-CAR was efficiently transduced into the purified CD8 + T cells to construct CAR-T cells. We evaluated the antitumor capability of the CD4-CAR transduced CD8 + T cells (anti-CD4 CD8-CAR T cells) both in vitro and in vivo . The anti-CD4 CD8-CAR T cells exhibited significant cancer-cell-killing capacity across multiple tumor cell lines, including CEM, Jurkat, and MV4-11. Meanwhile, anti-CD4 CD8-CAR T cells significantly inhibited tumor growth in vivo . In conclusion, the presented microfluidic chip offers a cost-effective and high-purity approach for CD8 + T cell separation, enhancing CD4-CAR transduction and achieving efficient antitumor capability both in vitro and in vivo .
Keyphrases
- cell therapy
- high throughput
- circulating tumor cells
- acute myeloid leukemia
- acute lymphoblastic leukemia
- nk cells
- gene expression
- stem cells
- mesenchymal stem cells
- endothelial cells
- gold nanoparticles
- mass spectrometry
- drug delivery
- liquid chromatography
- oxidative stress
- sensitive detection
- cancer therapy
- endoplasmic reticulum stress