Quantification of mutant SPOP proteins in prostate cancer using mass spectrometry-based targeted proteomics.

Hui WangChristopher E BarbieriJintang HeYuqian GaoTujin ShiChaochao WuAthena A SchepmoesThomas L FillmoreSung-Suk ChaeDennis HuangJuan Miguel MosqueraWei-Jun QianRichard D SmithSudhir SrivastavaJacob KaganDavid G CampKarin D RodlandMark A RubinTao Liu

Published in: Journal of translational medicine (2017)

In summary, PRISM-SRM enables multiplexed, isoform-specific detection of mutant SPOP proteins in cell lysates, providing significant potential in biomarker development for prostate cancer.

Keyphrases

prostate cancer
mass spectrometry
radical prostatectomy
single cell
liquid chromatography
label free
wild type
cell therapy
capillary electrophoresis
gas chromatography
stem cells
loop mediated isothermal amplification
drug delivery
risk assessment
bone marrow
sensitive detection