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DNA methylation profile of liver tissue in end-stage cholestatic liver disease.

Angela C CheungBrian D JuranErik M SchlichtBryan M McCauleyElizabeth J AtkinsonRaymond MooreJulie K HeimbachKymberly D WattTsung-Teh WuNicholas F LaRussoGregory J GoresZhifu SunKonstantinos N Lazaridis
Published in: Epigenomics (2022)
Aims: In this methylome-wide association study of cholestatic liver diseases (primary sclerosing cholangitis and primary biliary cholangitis), the authors aimed to elucidate changes in methylome and pathway enrichment to identify candidate genes . Patients & methods: Reduced representation bisulfite sequencing was performed on liver tissue from 58 patients with primary sclerosing cholangitis (n = 13), primary biliary cholangitis (n = 20), alcoholic liver disease (n = 21) and live liver donors (n = 4). Pathway enrichment and network analysis were used to explore key genes/pathways. Results: Both cholestatic liver diseases were characterized by global hypomethylation, with pathway enrichment demonstrating distinct genes and pathways associated with the methylome. Conclusions: This novel study demonstrated that differential methylation in cholestatic liver disease was associated with unique pathways, suggesting it may drive disease pathogenesis.
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